ChloroquineV1, Main, Exploration, bibRecord, 003282

Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Identifieur interne : 003282 ( Main/Exploration ); précédent : 003281; suivant : 003283

Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Auteurs : M. J. Tevethia [États-Unis] ; D. J. Spector [États-Unis] ; K. M. Leisure [États-Unis] ; M. F. Stinski [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1987.

RBID : ISTEX:5052016A37279CB3832A0846DC4F3E1EE7AA927D

English descriptors

Teeft :
- Acad, Adenovirus, Adenovirus mutants, Assay, Berk, Biol, Cells transfected, Chloramphenicol acetyltransferase, Complementation, Cytomegalovirus, Detectable activity, Digestion, Dna, Early gene regions, Early genes, Efficient complementation, Gene, Gene expression, Gene product, Gene products, Gene region, Gene regions, Genome, Hcmv, Hcmv gene, Hcmv gene regions, Hcmv genes, Herpes, Herpes simplex virus, Herpes simplex virus type, Human cells, Human cytomegalovirus, Hybridized, Infectious adenovirus, Infectious virus, Lane designations, Late adenovirus, Monkey cells, Mrna, Mutant, Nucleic acids, P2cat, Pcsdlacc, Pcsdlacc dnas, Plasmid, Proc, Promoter, Recombinant, Recombinant dnas, Replication, Rna, Simplex, Spector, Stenberg, Stinski, Tevethia, Transcription, Transcriptional, Transcriptional activation, Transfected, Viral, Viral genes.

Abstract

Abstract: The participation of human cytomegalovirus (HCMV) immediate early genes in the activation of the expression of adenovirus genes in trans (trans-activation) was examined. The initial strategy used was to determine the ability of HCMV genes to complement mutants of adenovirus El a, an immediate early gene which encodes a trans-activator. The HCMV immediate early gene regions IE1 and IE2 complemented E1a-deficient mutants in three separate assays. IE1 and IE2 substituted for E1 a in the synthesis of infectious adenovirus, late adenovirus RNA, and adenovirus DNA. Complementation by the IE2 gene region alone, but not by IE1 alone, was observed using the most discriminating assay, that for late adenovirus RNA synthesis. A role for both HCMV gene regions in positive transcriptional control was indicated by their ability to increase expression of chloramphenicol acetyltransferase (CAT) mediated by the adenovirus E2a promoter. The IE2 region alone activated CAT synthesis but lE1 alone had no detectable activity. Moreover, the activity of both gene regions was about 10-fold higher than that of IE2 alone. These data indicate that efficient complementation of E1a-deficient mutants and trans-activation of adenovirus early promoters involved the participation of both HCMV immediate early gene regions.

Url:

https://api.istex.fr/ark:/67375/6H6-HVLDCV94-4/fulltext.pdf

DOI: 10.1016/0042-6822(87)90119-X

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 002990
to stream Istex, to step Curation: 002990
to stream Istex, to step Checkpoint: 002040
to stream Main, to step Merge: 003354
to stream Main, to step Curation: 003282

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters</title>
<author><name sortKey="Tevethia, M J" sort="Tevethia, M J" uniqKey="Tevethia M" first="M. J." last="Tevethia">M. J. Tevethia</name>
</author>
<author><name sortKey="Spector, D J" sort="Spector, D J" uniqKey="Spector D" first="D. J." last="Spector">D. J. Spector</name>
</author>
<author><name sortKey="Leisure, K M" sort="Leisure, K M" uniqKey="Leisure K" first="K. M." last="Leisure">K. M. Leisure</name>
</author>
<author><name sortKey="Stinski, M F" sort="Stinski, M F" uniqKey="Stinski M" first="M. F." last="Stinski">M. F. Stinski</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:5052016A37279CB3832A0846DC4F3E1EE7AA927D</idno>
<date when="1987" year="1987">1987</date>
<idno type="doi">10.1016/0042-6822(87)90119-X</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-HVLDCV94-4/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002990</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002990</idno>
<idno type="wicri:Area/Istex/Curation">002990</idno>
<idno type="wicri:Area/Istex/Checkpoint">002040</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002040</idno>
<idno type="wicri:doubleKey">0042-6822:1987:Tevethia M:participation:of:two</idno>
<idno type="wicri:Area/Main/Merge">003354</idno>
<idno type="wicri:Area/Main/Curation">003282</idno>
<idno type="wicri:Area/Main/Exploration">003282</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters</title>
<author><name sortKey="Tevethia, M J" sort="Tevethia, M J" uniqKey="Tevethia M" first="M. J." last="Tevethia">M. J. Tevethia</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania 17033</wicri:regionArea>
<orgName type="university">Université d'État de Pennsylvanie</orgName>
<placeName><settlement type="city">University Park (Pennsylvanie)</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Spector, D J" sort="Spector, D J" uniqKey="Spector D" first="D. J." last="Spector">D. J. Spector</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania 17033</wicri:regionArea>
<orgName type="university">Université d'État de Pennsylvanie</orgName>
<placeName><settlement type="city">University Park (Pennsylvanie)</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Leisure, K M" sort="Leisure, K M" uniqKey="Leisure K" first="K. M." last="Leisure">K. M. Leisure</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania 17033</wicri:regionArea>
<orgName type="university">Université d'État de Pennsylvanie</orgName>
<placeName><settlement type="city">University Park (Pennsylvanie)</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stinski, M F" sort="Stinski, M F" uniqKey="Stinski M" first="M. F." last="Stinski">M. F. Stinski</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242</wicri:regionArea>
<orgName type="university">Université de l'Iowa</orgName>
<placeName><settlement type="city">Iowa City</settlement>
<region type="state">Iowa</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1987">1987</date>
<biblScope unit="volume">161</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="276">276</biblScope>
<biblScope unit="page" to="285">285</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term>
<term>Adenovirus</term>
<term>Adenovirus mutants</term>
<term>Assay</term>
<term>Berk</term>
<term>Biol</term>
<term>Cells transfected</term>
<term>Chloramphenicol acetyltransferase</term>
<term>Complementation</term>
<term>Cytomegalovirus</term>
<term>Detectable activity</term>
<term>Digestion</term>
<term>Dna</term>
<term>Early gene regions</term>
<term>Early genes</term>
<term>Efficient complementation</term>
<term>Gene</term>
<term>Gene expression</term>
<term>Gene product</term>
<term>Gene products</term>
<term>Gene region</term>
<term>Gene regions</term>
<term>Genome</term>
<term>Hcmv</term>
<term>Hcmv gene</term>
<term>Hcmv gene regions</term>
<term>Hcmv genes</term>
<term>Herpes</term>
<term>Herpes simplex virus</term>
<term>Herpes simplex virus type</term>
<term>Human cells</term>
<term>Human cytomegalovirus</term>
<term>Hybridized</term>
<term>Infectious adenovirus</term>
<term>Infectious virus</term>
<term>Lane designations</term>
<term>Late adenovirus</term>
<term>Monkey cells</term>
<term>Mrna</term>
<term>Mutant</term>
<term>Nucleic acids</term>
<term>P2cat</term>
<term>Pcsdlacc</term>
<term>Pcsdlacc dnas</term>
<term>Plasmid</term>
<term>Proc</term>
<term>Promoter</term>
<term>Recombinant</term>
<term>Recombinant dnas</term>
<term>Replication</term>
<term>Rna</term>
<term>Simplex</term>
<term>Spector</term>
<term>Stenberg</term>
<term>Stinski</term>
<term>Tevethia</term>
<term>Transcription</term>
<term>Transcriptional</term>
<term>Transcriptional activation</term>
<term>Transfected</term>
<term>Viral</term>
<term>Viral genes</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The participation of human cytomegalovirus (HCMV) immediate early genes in the activation of the expression of adenovirus genes in trans (trans-activation) was examined. The initial strategy used was to determine the ability of HCMV genes to complement mutants of adenovirus El a, an immediate early gene which encodes a trans-activator. The HCMV immediate early gene regions IE1 and IE2 complemented E1a-deficient mutants in three separate assays. IE1 and IE2 substituted for E1 a in the synthesis of infectious adenovirus, late adenovirus RNA, and adenovirus DNA. Complementation by the IE2 gene region alone, but not by IE1 alone, was observed using the most discriminating assay, that for late adenovirus RNA synthesis. A role for both HCMV gene regions in positive transcriptional control was indicated by their ability to increase expression of chloramphenicol acetyltransferase (CAT) mediated by the adenovirus E2a promoter. The IE2 region alone activated CAT synthesis but lE1 alone had no detectable activity. Moreover, the activity of both gene regions was about 10-fold higher than that of IE2 alone. These data indicate that efficient complementation of E1a-deficient mutants and trans-activation of adenovirus early promoters involved the participation of both HCMV immediate early gene regions.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Iowa</li>
<li>Pennsylvanie</li>
</region>
<settlement><li>Iowa City</li>
<li>University Park (Pennsylvanie)</li>
</settlement>
<orgName><li>Université d'État de Pennsylvanie</li>
<li>Université de l'Iowa</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Tevethia, M J" sort="Tevethia, M J" uniqKey="Tevethia M" first="M. J." last="Tevethia">M. J. Tevethia</name>
</region>
<name sortKey="Leisure, K M" sort="Leisure, K M" uniqKey="Leisure K" first="K. M." last="Leisure">K. M. Leisure</name>
<name sortKey="Spector, D J" sort="Spector, D J" uniqKey="Spector D" first="D. J." last="Spector">D. J. Spector</name>
<name sortKey="Stinski, M F" sort="Stinski, M F" uniqKey="Stinski M" first="M. F." last="Stinski">M. F. Stinski</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003282 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003282 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    ChloroquineV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:5052016A37279CB3832A0846DC4F3E1EE7AA927D
   |texte=   Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters
}}

This area was generated with Dilib version V0.6.33.
Data generation: Wed Mar 25 22:43:59 2020. Site generation: Sun Jan 31 12:44:45 2021

	Serveur d'exploration Chloroquine
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Chloroquine

Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri